IWRFRI'2000

Destroying basidiomycetes as producer of lignolitic enzymes
Belova N.V., Gavrilova V.P.
Komarov Botanical Institute RAS, 197376 St Petersburg, Russia

For a long time the Culture Collection of Basidiomycetes of Komarov Botanical Institute- LE(BIN), maintains more than 1200 living tissue isolates of macrofungi from Russia. Collection contains the database, which includes information about nomenclatural data and strains characters.

Many strains are approved in different biotechnological projects. This is connected with possibility of mushrooms to produce many extracellular hydrolytic and oxidizing enzymes during fermentation on liquid culture media. Wood decay basidiomycetes are the most interesting group between fungi. The collection contains more than 100 strains of this kind of fungi, which produce lignolytic enzymes, such as peroxidases and phenoloxidases. Basidiomycetes enzymes have high activity and thermal stability and can be used in pure state as well in complex from cultural filtrates. The last can contain different ratio of enzymes, which depends on the fermentation conditions. Receiving of these enzyme complexes doesn't need additional purification. These complexes are more cheep and effective than pure enzymes. Mushroom enzyme complex and native enzymes can be used for removing lignincontaining admixtures, bleaching textile fibres and improving their properties.

The cultures of species Coriolus hirsutus, Coriolus versicolor, Coriolus zonatus, Cerrena maxima and Cerrena unicolor are offered as producers of lignolytic enzyme complexes and native enzymes (laccase and peroxidase).

Reference

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